SlrA

• Description: anti-repressor, antagonist of SinR and SlrR, involved in control of flagellar genes and eps and tapA-sipW-tasA operons
  
  

Gene name	slrA
Synonyms
Essential	no
Product	antagonist of SinR and SlrR
Function	control of biofilm formation
Gene expression levels in SubtiExpress: slrA
Interactions involving this protein in SubtInteract: SlrA
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	6.1 kDa, 9.992
Gene length, protein length	156 bp, 52 aa
Immediate neighbours	ywcC, ywcB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

YwcC regulon

The gene

Basic information

• Locus tag: BSU38229

Database entries

• DBTBS entry: no entry

• SubtiList entry:

Additional information

• This gene was only recently identified. PubMed
• SlrA overexpression results in reduced expression of the SigD regulon and in enhanced expression of the SinR regulon, this can be suppressed by inactivation of either remA, remB, slrR, or sinR PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding of SinR resulting in induction of SinR-repressed genes and operons PubMed

• Protein family:

• Paralogous protein(s): SinI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: SlrR inhibits activity of SlrA (i. e. binding to SinR) PubMed

• Interactions:
  • SlrA-SinR PubMed, K(D) 10.6 nM PubMed
  • SlrA-SlrR PubMed

• Localization:

Database entries

• Structure:

• UniProt: P0C8M5

• KEGG entry: no entry

• E.C. number:

Expression and regulation

• Operon:
  • slrA-ywcB-ywcA
  • slrA-ywcB
  • slrA

• Expression browser: slrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • YwcC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Additional publications: PubMed

Joseph A Newman, Cecilia Rodrigues, Richard J Lewis
Molecular basis of the activity of SinR protein, the master regulator of biofilm formation in Bacillus subtilis.
J Biol Chem: 2013, 288(15);10766-78
[PubMed:23430750] [WorldCat.org] [DOI] (I p)

Yunrong Chai, Roberto Kolter, Richard Losick
Paralogous antirepressors acting on the master regulator for biofilm formation in Bacillus subtilis.
Mol Microbiol: 2009, 74(4);876-87
[PubMed:19788541] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
SlrR/SlrA controls the initiation of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 69(6);1399-410
[PubMed:18647168] [WorldCat.org] [DOI] (I p)