CwlC

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  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlC
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function mother cell lysis
MW, pI 27 kDa, 5.939
Gene length, protein length 765 bp, 255 aa
Immediate neighbours ymaB, spoVK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CwlC context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

cell wall/ other, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU17410

Expression

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1X60 (peptidoglycan binding domain)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Masaki Mishima, Toshio Shida, Kazuto Yabuki, Ken-Ichi Kato, Junichi Sekiguchi, Chojiro Kojima
Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR.
Biochemistry: 2005, 44(30);10153-63
[PubMed:16042392] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
J Biol Chem: 2001, 276(30);28140-6
[PubMed:11375403] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

A Kuroda, Y Asami, J Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
J Bacteriol: 1993, 175(19);6260-8
[PubMed:8407798] [WorldCat.org] [DOI] (P p)