Methods

From SubtiWiki
Revision as of 18:44, 16 August 2011 by Jstuelk (talk | contribs)
Jump to: navigation, search

Here you can find a list of methods for working with Bacillus subtilis:

  • Bacterial Two Hybrid: BACTH
  • introduction of markerfree deletions: PubMed
  • Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
  • transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
  • Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
  • ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
  • Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed

Imke G de Jong, Katrin Beilharz, Oscar P Kuipers, Jan-Willem Veening
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy.
J Vis Exp: 2011, (53);
[PubMed:21841760] [WorldCat.org] [DOI] (I e)

Chong Zhang, Xiaohui Zhang, Zhengying Yao, Yaping Lu, Fengxia Lu, Zhaoxin Lu
A new method for multiple gene inactivations in Bacillus subtilis 168, producing a strain free of selectable markers.
Can J Microbiol: 2011, 57(5);427-36
[PubMed:21542786] [WorldCat.org] [DOI] (I p)

Reindert Nijland, J Grant Burgess, Jeff Errington, Jan-Willem Veening
Transformation of environmental Bacillus subtilis isolates by transiently inducing genetic competence.
PLoS One: 2010, 5(3);e9724
[PubMed:20300532] [WorldCat.org] [DOI] (I e)

Haojie Yu, Xin Yan, Weiliang Shen, Yujia Shen, Ji Zhang, Shunpeng Li
Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome.
J Microbiol Biotechnol: 2010, 20(1);45-53
[PubMed:20134232] [WorldCat.org] (P p)

Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara
A new simple method to introduce marker-free deletions in the Bacillus subtilis genome.
Genes Genet Syst: 2009, 84(4);315-8
[PubMed:20057169] [WorldCat.org] [DOI] (P p)

A Nicole Edwards, Jason D Fowlkes, Elizabeth T Owens, Robert F Standaert, Dale A Pelletier, Gregory B Hurst, Mitchel J Doktycz, Jennifer L Morrell-Falvey
An in vivo imaging-based assay for detecting protein interactions over a wide range of binding affinities.
Anal Biochem: 2009, 395(2);166-77
[PubMed:19698693] [WorldCat.org] [DOI] (I p)

Shu Ishikawa, Yoshitoshi Ogura, Mika Yoshimura, Hajime Okumura, Eunha Cho, Yoshikazu Kawai, Ken Kurokawa, Taku Oshima, Naotake Ogasawara
Distribution of stable DnaA-binding sites on the Bacillus subtilis genome detected using a modified ChIP-chip method.
DNA Res: 2007, 14(4);155-68
[PubMed:17932079] [WorldCat.org] [DOI] (P p)