SpoIVCB

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Gene name spoIVCB
Synonyms cisB, sigK
Essential no
Product RNA polymerase sporulation-specific sigma factor
(sigma-K) (N-terminal half)
Function late mother cell-specific gene expression
Interactions involving this protein in SubtInteract: SigK
MW, pI 17 kDa, 8.208
Gene length, protein length 468 bp, 156 aa
Immediate neighbours nucB, spoIVCA
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
SpoIVCB context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon, SigK regulon, SpoIIID regulon

The SigK regulon

The gene

Basic information

  • Locus tag: BSU25760

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • SigK is activated by processing of membrane-bound pro-SigK by the SpoIVFB metalloprotease PubMed
    • spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigK regulon

Takeko Kodama, Takeshi Matsubayashi, Tadayoshi Yanagihara, Hiroyuki Komoto, Katsutoshi Ara, Katsuya Ozaki, Ritsuko Kuwana, Daisuke Imamura, Hiromu Takamatsu, Kazuhito Watabe, Junichi Sekiguchi
A novel small protein of Bacillus subtilis involved in spore germination and spore coat assembly.
Biosci Biotechnol Biochem: 2011, 75(6);1119-28
[PubMed:21670523] [WorldCat.org] [DOI] (I p)

Jessica M Silvaggi, John B Perkins, Richard Losick
Genes for small, noncoding RNAs under sporulation control in Bacillus subtilis.
J Bacteriol: 2006, 188(2);532-41
[PubMed:16385044] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)


Original Publications