SucC

• Description: succinyl-CoA synthetase (beta subunit)
  
  

Gene name	sucC
Synonyms
Essential	no
Product	succinyl-CoA synthetase (beta subunit)
Function	TCA cycle
Interactions involving this protein in SubtInteract: SucC
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	41 kDa, 4.846
Gene length, protein length	1155 bp, 385 aa
Immediate neighbours	ylqH, sucD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU16090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)

• Protein family: ATP-grasp domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible Michaelis-Menten FEBS Letters

• Domains:

• Modification: phosphorylation on Ser-220 PubMed

• Cofactor(s):

• Effectors of protein activity:
  • Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
    • GTP is not accept by the enzyme FEBS Letters

• Interactions:
  • Mdh-SucC PubMed
  • SucC-SucD PubMed

• Localization:

Database entries

• Structure: 1JKJ (E. coli)

• UniProt: P80886

• KEGG entry: [3]

• E.C. number: 6.2.1.5

Additional information

• extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia

Expression and regulation

• Operon: sucC-sucD PubMed

• Sigma factor:

• Regulation: repressed by glucose (2.7-fold) (CcpA) PubMed

• Regulatory mechanism: CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: