PGP1389

From SubtiWiki
Revision as of 19:05, 18 October 2010 by Jstuelk (talk | contribs)
Jump to: navigation, search
pGP1331: click to enlarge

General comments

Integrative plasmid for the fusion of the C-terminus of a protein to a Streptag tag, keeping the control of expression under control of the natural promotor of the target gene.

  • E.coli: multicopy plasmid, select for ampicillin
  • B. subtilis: no ori, select for spectinomycin

Hints for the utilisation of the plasmid

  • amplify the last 600bp of your gene of interest,
  • skip the Stopp-Codon of the gene in the reverse primer
  • take care that the integration is in-frame