SepF

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  • Description: part of the divisome

Gene name sepF
Synonyms ylmF
Essential no
Product FtsZ-interacting protein
Function proper execution of septum synthesis
MW, pI 17 kDa, 4.863
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ylmE, ylmG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YlmF context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU15390

Phenotypes of a mutant

Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Cell division

  • Protein family: sepF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, CBCB, Newcastle University, UK

Shu Ishikawa, Nara Institute of Science and Technology, Nara, Japan

Your additional remarks

SepF mutation is synthetic lethal in combination with an ezrA mutation or an ftsA mutation.

References

Reviews

David W Adams, Jeff Errington
Bacterial cell division: assembly, maintenance and disassembly of the Z ring.
Nat Rev Microbiol: 2009, 7(9);642-53
[PubMed:19680248] [WorldCat.org] [DOI] (I p)


Original Publications