BglC

• Description: endo-1,4-beta-glucanase
  
  

Gene name	bglC
Synonyms	eglS
Essential	no
Product	endo-1,4-beta-glucanase)
Function	beta-1,4-glucan degradation
MW, pI	55 kDa, 8.619
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	alsT, ynfE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 5 (cellulase A) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 1H2J (from Bacillus agaradhaerens)

• UniProt: P10475

• KEGG entry: [3]

• E.C. number: 3.2.1.4

Expression and regulation

• Operon: bglC (according to DBTBS)

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

L M Robson, G H Chambliss
Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.
J Bacteriol: 1987, 169(5);2017-25
[PubMed:3106328] [WorldCat.org] [DOI] (P p)

R M MacKay, A Lo, G Willick, M Zuker, S Baird, M Dove, F Moranelli, V Seligy
Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene.
Nucleic Acids Res: 1986, 14(22);9159-70
[PubMed:3024130] [WorldCat.org] [DOI] (P p)