YkuI

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  • Description: c-di-GMP specific phosphodiesterase

Gene name ykuI
Synonyms
Essential no
Product c-di-GMP specific phosphodiesterase
Function unknown
MW, pI 47 kDa, 4.462
Gene length, protein length 1221 bp, 407 aa
Immediate neighbours ykuH, fsrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkuI context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU14090

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251] [WorldCat.org] [DOI] (P p)