Ldh

• Description: L-lactate dehydrogenase
  
  

Gene name	ldh
Synonyms	lctE
Essential	no
Product	L-lactate dehydrogenase
Function	overflow metabolism, fermentation
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 5.394
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	amyE, lctP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-lactate + NAD⁺ = pyruvate + NADH (according to Swiss-Prot)

• Protein family: LDH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Tyr-224 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1LDN (+) and fructose-1.6-bisphosphate, Geobacillus stearothermophilus), 2LDB (Geobacillus stearothermophilus)

• UniProt: P13714

• KEGG entry: [3]

• E.C. number: 1.1.1.27

Expression and regulation

• Operon: ldh-lctP PubMed

• Sigma factor:

• Regulation: induced under anaerobic conditions (Rex) PubMed

• Regulatory mechanism:
  • Rex: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Susana Romero, Enrique Merino, Francisco Bolívar, Guillermo Gosset, Alfredo Martinez
Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolism.
Appl Environ Microbiol: 2007, 73(16);5190-8
[PubMed:17586670] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915] [WorldCat.org] [DOI] (P p)