GabP

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  • Description: gamma-amino butyric acid permease and minor proline permease

Gene name gabP
Synonyms nrg-21
Essential no
Product gamma-amino butyric acid permease
Function utilization of gamma-amino butyric acid
Gene expression levels in SubtiExpress: gabP
Metabolic function and regulation of this protein in SubtiPathways:
Ala, Gly, Ser
MW, pI 50 kDa, 9.45
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours cotA, ydzX
Sequences Protein DNA DNA_with_flanks
Genetic context
GabP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GabP expression.png















Categories containing this gene/protein

transporters/ other, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU06310

Phenotypes of a mutant

  • defective in the utilization of gamma-amino butyrate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: secondary amino acid transporter

Extended information on the protein

  • Kinetic information:
    • K(M) for gamma-amino butyrate: 77 +/- 17 µM PubMed
    • K(M) for proline: 700 +/- 330 µM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252] [WorldCat.org] [DOI] (I p)

C E Brechtel, S C King
4-Aminobutyrate (GABA) transporters from the amine-polyamine-choline superfamily: substrate specificity and ligand recognition profile of the 4-aminobutyrate permease from Bacillus subtilis.
Biochem J: 1998, 333 ( Pt 3)(Pt 3);565-71
[PubMed:9677314] [WorldCat.org] [DOI] (P p)

A E Ferson, L V Wray, S H Fisher
Expression of the Bacillus subtilis gabP gene is regulated independently in response to nitrogen and amino acid availability.
Mol Microbiol: 1996, 22(4);693-701
[PubMed:8951816] [WorldCat.org] [DOI] (P p)