Fni

From SubtiWiki
Revision as of 10:35, 14 May 2013 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: isopentenyl diphosphate isomerase

Gene name fni
Synonyms ypgA
Essential no
Product isopentenyl diphosphate isomerase
Function lipid metabolism
Gene expression levels in SubtiExpress: fni
MW, pI 22 kDa, 6.101
Gene length, protein length 636 bp, 212 aa
Immediate neighbours ypzI, ypfD
Sequences Protein DNA DNA_with_flanks
Genetic context
YpgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Fni expression.png
























Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22870

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)
  • Protein family: IPP isomerase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1P0N (complex with FMN), 1P0K
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175] [WorldCat.org] [DOI] (P p)

Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687] [WorldCat.org] [DOI] (P p)