MifM

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  • Description: ribosome-nascent chain sensor of membrane protein biogenesis

Gene name mifM
Synonyms yqzJ
Essential no
Product sensor of SpoIIIJ activity
Function control of yidC2 translation
Gene expression levels in SubtiExpress: mifM
Interactions involving this protein in SubtInteract: MifM
MW, pI 11 kDa, 5.829
Gene length, protein length 285 bp, 95 aa
Immediate neighbours polY1, yidC2
Sequences Protein DNA Advanced_DNA
Genetic context
YqzJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MifM expression.png















Categories containing this gene/protein

translation, membrane proteins

This gene is a member of the following regulons

The MifM regulon: yidC2

The gene

Basic information

  • Locus tag: BSU23880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal transmembrane domain PubMed
    • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • An antisense RNA is predicted for mifM PubMed
    • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
    • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References