ComGB

• Description: polytopic membrane protein , DNA transport machinery
  
  

Gene name	comGB
Synonyms
Essential	no
Product	DNA transport machinery
Function	genetic competence, DNA uptake
Interactions involving this protein in SubtInteract: ComGB
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	36 kDa, 8.654
Gene length, protein length	969 bp, 323 aa
Immediate neighbours	comGC, comGA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: GSP F family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGB-ComGA PubMed
  • ComGB-ComGC PubMed
  • ComGB-ComGD PubMed
  • ComGB-ComGE PubMed
  • ComGB-ComGG PubMed

• Localization: cell membrane, polar localization PubMed

Database entries

• Structure:

• UniProt: P25954

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

Y S Chung, D Dubnau
All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.
J Bacteriol: 1998, 180(1);41-5
[PubMed:9422590] [WorldCat.org] [DOI] (P p)

D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol Microbiol: 1994, 11(4);695-703
[PubMed:8196543] [WorldCat.org] [DOI] (P p)

M Albano, R Breitling, D A Dubnau
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon.
J Bacteriol: 1989, 171(10);5386-404
[PubMed:2507524] [WorldCat.org] [DOI] (P p)