AmyX

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  • Description: pullulanase (debranching enzyme)

Gene name amyX
Synonyms
Essential no
Product pullulanase
Function starch degradation
Gene expression levels in SubtiExpress: amyX
MW, pI 80 kDa, 5.799
Gene length, protein length 2154 bp, 718 aa
Immediate neighbours ytmP, ytlR
Sequences Protein DNA DNA_with_flanks
Genetic context
AmyX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AmyX expression.png
























Categories containing this gene/protein

utilization of specific carbon sources, sporulation proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): GlgB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2E8Z (complex with alpha cyclodextrin), 2E9B (complex with maltose)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jae-Hoon Shim, Jong-Tae Park, Jung-Sun Hong, Ki Woo Kim, Myo-Jeong Kim, Jung-Hyuk Auh, Young-Wan Kim, Cheon-Seok Park, Winfried Boos, Jung-Wan Kim, Kwan-Hwa Park
Role of maltogenic amylase and pullulanase in maltodextrin and glycogen metabolism of Bacillus subtilis 168.
J Bacteriol: 2009, 191(15);4835-44
[PubMed:19465663] [WorldCat.org] [DOI] (I p)

Dominggus Malle, Takafumi Itoh, Wataru Hashimoto, Kousaku Murata, Shigeru Utsumi, Bunzo Mikami
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 4);381-4
[PubMed:16582490] [WorldCat.org] [DOI] (I p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)