DefB

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  • Description: N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine

Gene name defB
Synonyms ykrB
Essential no
Product N-formylcysteine deformylase
Function utilization of S-methyl-cysteine
Gene expression levels in SubtiExpress: defB
MW, pI 20 kDa, 5.456
Gene length, protein length 552 bp, 184 aa
Immediate neighbours ykrA, ykzV
Sequences Protein DNA DNA_with_flanks
Genetic context
YkrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkrB expression.png















Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14560

Phenotypes of a mutant

  • no growth with S-methyl cysteine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N-acetylcysteine --> cysteine + acetate PubMed
  • Protein family: polypeptide deformylase family (according to Swiss-Prot)
  • Paralogous protein(s): DefA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 580 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 735 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997] [WorldCat.org] [DOI] (I p)

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)