Fni

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  • Description: isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis

Gene name fni
Synonyms ypgA, idi
Essential no
Product isopentenyl diphosphate isomerase
Function MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: fni
MW, pI 22 kDa, 6.101
Gene length, protein length 636 bp, 212 aa
Immediate neighbours ypzI, ypfD
Sequences Protein DNA DNA_with_flanks
Genetic context
YpgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Fni expression.png















Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22870

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)
  • Protein family: IPP isomerase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175] [WorldCat.org] [DOI] (P p)

Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687] [WorldCat.org] [DOI] (P p)