HxlA

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  • Description: 3-hexulose-6-phosphate synthase

Gene name hxlA
Synonyms yckG
Essential no
Product 3-hexulose-6-phosphate synthase
Function ribulose monophosphate pathway for formaldehyde fixation
Gene expression levels in SubtiExpress: hxlA
MW, pI 22 kDa, 4.564
Gene length, protein length 630 bp, 210 aa
Immediate neighbours hxlB, hxlR
Sequences Protein DNA Advanced_DNA
Genetic context
HxlA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HxlA expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HxlR regulon

The gene

Basic information

  • Locus tag: BSU03460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde (according to Swiss-Prot)
  • Protein family: HPS subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induction by formaldehyde (HxlR) PubMed
  • Regulatory mechanism: transcription activation by HxlR PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Nobuo Kato, Hiroya Yurimoto, Rudolf K Thauer
The physiological role of the ribulose monophosphate pathway in bacteria and archaea.
Biosci Biotechnol Biochem: 2006, 70(1);10-21
[PubMed:16428816] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)