RocB

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  • Description: involved in arginine and ornithine utilization

Gene name rocB
Synonyms ipa-77d
Essential no
Product unknown
Function arginine, ornithine and citrulline utilization
Gene expression levels in SubtiExpress: rocB
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 64 kDa, 5.347
Gene length, protein length 1698 bp, 566 aa
Immediate neighbours rocC, rocA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RocB expression.png




























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU37770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YqjN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induction by arginine (RocR, AhrC) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

U Klingel, C M Miller, A K North, P G Stockley, S Baumberg
A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/activator of arginine metabolism.
Mol Gen Genet: 1995, 248(3);329-40
[PubMed:7565595] [WorldCat.org] [DOI] (P p)

S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162] [WorldCat.org] [DOI] (P p)