LeuB

From SubtiWiki
Revision as of 10:37, 26 October 2010 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: 3-isopropylmalate dehydrogenase

Gene name leuB
Synonyms leuC
Essential no
Product 3-isopropylmalate dehydrogenase
Function biosynthesis of leucine
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val, Coenzyme A
MW, pI 39 kDa, 4.744
Gene length, protein length 1095 bp, 365 aa
Immediate neighbours leuC, leuA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LeuB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU28270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH (according to Swiss-Prot)
  • Protein family: LeuB type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1XAD (Thermus thermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References