Difference between revisions of "Sandbox"

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−
* '''Description:''' undecaprenyl pyrophosphate phosphatase, bacitracin resistance <br/><br/>
+
* '''Description:''' phospho-beta-glucosidase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''bcrC''
+
|''bglH''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywoA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || undecaprenyl pyrophosphate phosphatase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-beta-glucosidase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to bacitracin and oxidative stress
+
|style="background:#ABCDEF;" align="center"|'''Function''' || salicin utilization
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 9.455  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.957  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1407 bp, 469 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrgB]]'', ''[[ywnJ]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxiE]]'', ''[[bglP]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15670&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15962&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:ywoA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:bglH_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU36530
+
* '''Locus tag:''' BSU39260
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12488]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10935]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H<sub>2</sub>O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)
  
−
* '''Protein family:''' bcrC/ybjG/ywoA family (according to Swiss-Prot)
+
* '''Protein family:''' glycosyl hydrolase 1 family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94571 P94571]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P40740 P40740]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU36530]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39260]
  
−
* '''E.C. number:'''
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.86 3.2.1.86]
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''bcrC''
+
* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
  
−
* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed], [[SigI]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/18156261 PubMed]
+
* '''[[Sigma factor]]:''' [[SigA]]
  
−
* '''Regulation:''' expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
+
* '''Regulation:''' repressed by glucose ([[CcpA]]) ,  induced by salicin  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed], repressed by glucose (catabolite repression)
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression,  Induction: [[LicT]]-dependent RNA switch (antitermination), catabolite repression: repression by [[CcpA]]  ([[CcpA]] binding site overlaps -35 region) and lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 118: Line 118:
 
=References=
 
=References=
  
−
<pubmed>12850135 17434969 18156261, </pubmed>
+
<pubmed>7883710 7559347, </pubmed>
−
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
+
 
−
# Jervis et al. 2007. SigM-responsive genes of ''Bacillus subtilis'' and their promoters. J. Bacteriol. 189: 4534-4538. [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed]
+
# Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in ''Bacillus subtilis'': The ''bglP'' gene product has both transport and regulatory functions, similar to that of the ''Escherichia coli'' BglF protein. J. Bacteriol. 177: 1527-1535. [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
−
# Tseng, C. L., and Shaw, G. C. (2008) Genetic evidence for the actin homolog gene ''mreBH'' and the bacitracin resistance gene ''bcrC'' as targets of the alternative [[sigma factor]] SigI of ''Bacillus subtilis''. ''J. Bacteriol.'' '''190:''' 1561-1567. [http://www.ncbi.nlm.nih.gov/sites/entrez/18156261 PubMed]
+
# Krüger, S. and Hecker, M. (1995) Regulation of the putative ''bglPH'' operon for aryl-ß-glycoside utilization in ''Bacillus subtilis''. J. Bacteriol. 177, 5590-5597. [http://www.ncbi.nlm.nih.gov/sites/entrez/7559347 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:10, 8 June 2009

  • Description: phospho-beta-glucosidase

Gene name bglH
Synonyms
Essential no
Product phospho-beta-glucosidase
Function salicin utilization
MW, pI 53 kDa, 4.957
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours yxiE, bglP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BglH context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU39260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , induced by salicin PubMed, repressed by glucose (catabolite repression)
  • Regulatory mechanism: CcpA: transcription repression, Induction: LicT-dependent RNA switch (antitermination), catabolite repression: repression by CcpA (CcpA binding site overlaps -35 region) and lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

S Krüger, M Hecker
Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5590-7
[PubMed:7559347] [WorldCat.org] [DOI] (P p)

D Le Coq, C Lindner, S Krüger, M Steinmetz, J Stülke
New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog.
J Bacteriol: 1995, 177(6);1527-35
[PubMed:7883710] [WorldCat.org] [DOI] (P p)


  1. Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in Bacillus subtilis: The bglP gene product has both transport and regulatory functions, similar to that of the Escherichia coli BglF protein. J. Bacteriol. 177: 1527-1535. PubMed
  2. Krüger, S. and Hecker, M. (1995) Regulation of the putative bglPH operon for aryl-ß-glycoside utilization in Bacillus subtilis. J. Bacteriol. 177, 5590-5597. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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