Difference between revisions of "PWH844"

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[[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']]
 
[[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']]
  
pWH844
 
  
 
vector for the expression of proteins in ''E. coli'', the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
 
vector for the expression of proteins in ''E. coli'', the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

Revision as of 11:30, 16 April 2009

pWH844: click to enlarge
pWH844 cloning region: click to enlarge


vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Hillen lab

in E. coli: ampicillin resistance

similar plasmid: pGP570

sequencing primer:

  • JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
  • SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
  • PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
  • pWH844 fw: 5’-TATGAGAGGATCGCATCACCAT-3‘


Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed