Difference between revisions of "TopB"

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(Basic information/ Evolution)
(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
 
** BKE04260 (''topB''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [[Jörg Stülke]]'s lab)
 
** BKE04260 (''topB''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [[Jörg Stülke]]'s lab)
 +
** GP1864 (''topB''::''ermC'') (available in [[Jörg Stülke]]'s lab)
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:04, 3 August 2015

  • Description: DNA topoisomerase III

Gene name topB
Synonyms
Essential no
Product DNA topoisomerase III
Function replication
Gene expression levels in SubtiExpress: topB
MW, pI 81 kDa, 9.879
Gene length, protein length 2181 bp, 727 aa
Immediate neighbours lrpC, ydaJ
Sequences Protein DNA DNA_with_flanks
Genetic context
TopB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TopB expression.png















Categories containing this gene/protein

DNA replication, DNA condensation/ segregation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU04260

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s): TopA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 144 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 452 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)