Difference between revisions of "PghL"
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|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''pghL'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yndL '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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|style="background:#ABCDEF;" align="center"|'''Function''' || polyglutamic acid degradation | |style="background:#ABCDEF;" align="center"|'''Function''' || polyglutamic acid degradation | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17820 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17820 pghL] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.92 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.92 | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' '' | + | * '''Operon:''' ''pghL'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16385044 PubMed] |
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yndL_1915221_1915979_1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yndL_1915221_1915979_1 pghL] {{PubMed|22383849}} |
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190,16385044}} | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190,16385044}} | ||
| − | * '''Regulation:''' expressed during sporulation in the mother cell[http://www.ncbi.nlm.nih.gov/sites/entrez/16385044 PubMed] | + | * '''Regulation:''' expressed during [[sporulation]] in the mother cell[http://www.ncbi.nlm.nih.gov/sites/entrez/16385044 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| − | ** An [[ncRNA|antisense RNA]], ''[[surA]]'', is predicted for ''[[ | + | ** An [[ncRNA|antisense RNA]], ''[[surA]]'', is predicted for ''[[pghL]]'' {{PubMed|20525796}} |
=Biological materials = | =Biological materials = | ||
Revision as of 08:55, 13 July 2015
- Description: phage-derived gamma polyglutamic acid hydrolase, sporulation protein
| Gene name | pghL |
| Synonyms | yndL |
| Essential | no |
| Product | phage-derived gamma polyglutamic acid hydrolase |
| Function | polyglutamic acid degradation |
| Gene expression levels in SubtiExpress: pghL | |
| MW, pI | 28 kDa, 6.92 |
| Gene length, protein length | 756 bp, 252 aa |
| Immediate neighbours | surA, yndM |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
capsule biosynthesis and degradation, sporulation proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17820
Phenotypes of a mutant
Database entries
- BsubCyc: BSU17820
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- hyhrolysis of polyglutamic acid PubMed
- Protein family: UPF0714 family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains: DUF867 [3], gamma-PGA hydrolase domain PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU17820
- Structure:
- UniProt: O31815
- KEGG entry: [4]
- E.C. number:
Additional information
Expression and regulation
- Operon: pghL PubMed
- Regulation: expressed during sporulation in the mother cellPubMed
- Regulatory mechanism:
- Additional information:
- An antisense RNA, surA, is predicted for pghL PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Stefania Mamberti, Paola Prati, Paolo Cremaschi, Claudio Seppi, Carlo F Morelli, Alessandro Galizzi, Massimo Fabbi, Cinzia Calvio
γ-PGA Hydrolases of Phage Origin in Bacillus subtilis and Other Microbial Genomes.
PLoS One: 2015, 10(7);e0130810
[PubMed:26158264]
[WorldCat.org]
[DOI]
(I e)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Jessica M Silvaggi, John B Perkins, Richard Losick
Genes for small, noncoding RNAs under sporulation control in Bacillus subtilis.
J Bacteriol: 2006, 188(2);532-41
[PubMed:16385044]
[WorldCat.org]
[DOI]
(P p)
