Difference between revisions of "TufA"

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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:31, 16 February 2015

  • Description: elongation factor Tu

Gene name tufA
Synonyms
Essential yes PubMed
Product elongation factor Tu
Function translation
Gene expression levels in SubtiExpress: tufA
Interactions involving this protein in SubtInteract: TufA
MW, pI 43 kDa, 4.723
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours fusA, ybaC
Sequences Protein DNA DNA_with_flanks
Genetic context
TufA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TufA expression.png















Categories containing this gene/protein

translation, essential genes, membrane proteins, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01130

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: EF-Tu/EF-1A subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Thr-384 by PrkC, dephosphorylated by PrpC PubMed, PubMed
    • Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species PubMed
    • phosphorylated on several Arg residues PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 59691 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 237848 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 102596 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 40568 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 44607 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP839, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP847, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References