Difference between revisions of "YvcL"

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(Biological materials)
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* '''Mutant:''' GP231 (''amyE::P[[rpsJ]]-lacZ, yvcL::cat'') available in [[ Jörg Stülke]]'s lab; plasmid pGP1468 was used to disrupt the gene.
 
* '''Mutant:''' GP231 (''amyE::P[[rpsJ]]-lacZ, yvcL::cat'') available in [[ Jörg Stülke]]'s lab; plasmid pGP1468 was used to disrupt the gene.
  
* '''Expression vector:''' pGP1469 for expression in ''B. subtilis'' (in [[pGP382]], with C-terminal STREP-TAG), available in [[ Jörg Stülke]]'s lab
+
* '''Expression vector:'''  
 +
** pGP1469 for expression in ''B. subtilis'' (in [[pGP382]], with C-terminal STREP-TAG), available in [[ Jörg Stülke]]'s lab
 +
** pGP2429 (N-terminal Strep-tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [[SPINE]], available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 14:20, 8 December 2014

  • Description: WhiA family protein, involved in Z ring assembly, putative transcription factor

Gene name yvcL
Synonyms
Essential no
Product cell division protein
Function control of Z ring asembly
Gene expression levels in SubtiExpress: yvcL
MW, pI 36 kDa, 9.479
Gene length, protein length 948 bp, 316 aa
Immediate neighbours crh, yvcK
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcL expression.png















Categories containing this gene/protein

cell division, transcription factors and their control

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34750

Phenotypes of a mutant

  • a yvcL zapA double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) PubMed
  • a yvcL ezrA double mutant grows poorly, and the cells are filamentous PubMed
  • a yvcL minC-minD mutant grows poorly, and the cells are filamentous PubMed
  • a yvcL noc double mutant grows poorly, and the cells are filamentous PubMed
  • the defects of the yvcL zapA double mutant can be suppressed by inactivation of either gtaB, pgcA, or ugtP PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: WhiA family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3HYI (Crystal structure of full-length DUF199/WhiA from Thermotoga maritima)
    • 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • constitutive expression at both protein and RNA levels PubMed
    • very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.
  • Expression vector:
    • pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab
    • pGP2429 (N-terminal Strep-tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:

Labs working on this gene/protein

Your additional remarks

WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).

References

Katarina Surdova, Pamela Gamba, Dennis Claessen, Tjalling Siersma, Martijs J Jonker, Jeff Errington, Leendert W Hamoen
The conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis.
J Bacteriol: 2013, 195(24);5450-60
[PubMed:24097947] [WorldCat.org] [DOI] (I p)

Brett K Kaiser, Matthew C Clifton, Betty W Shen, Barry L Stoddard
The structure of a bacterial DUF199/WhiA protein: domestication of an invasive endonuclease.
Structure: 2009, 17(10);1368-76
[PubMed:19836336] [WorldCat.org] [DOI] (I p)

Lukasz Knizewski, Krzysztof Ginalski
Bacterial DUF199/COG1481 proteins including sporulation regulator WhiA are distant homologs of LAGLIDADG homing endonucleases that retained only DNA binding.
Cell Cycle: 2007, 6(13);1666-70
[PubMed:17603302] [WorldCat.org] [DOI] (I p)

Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399] [WorldCat.org] [DOI] (P p)

J A Aínsa, N J Ryding, N Hartley, K C Findlay, C J Bruton, K F Chater
WhiA, a protein of unknown function conserved among gram-positive bacteria, is essential for sporulation in Streptomyces coelicolor A3(2).
J Bacteriol: 2000, 182(19);5470-8
[PubMed:10986251] [WorldCat.org] [DOI] (P p)

A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995] [WorldCat.org] [DOI] (P p)