Difference between revisions of "NrgB"

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(Reviews)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP259 (cat), GP255 (nrgAB, cat), both available in the [[Stülke]] lab
+
* '''Mutant:''' GP259 (cat), GP255 (''[[nrgA]]-[[nrgB]]'', cat), both available in [[Jörg Stülke]]'s lab {{PubMed|14600241}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:15, 21 May 2014

  • Description: nitrogen-regulated PII-like protein

Gene name nrgB
Synonyms glnK
Essential no
Product nitrogen-regulated PII-like protein
Function regulation of ammonium uptake
Gene expression levels in SubtiExpress: nrgB
Interactions involving this protein in SubtInteract: NrgB
Metabolic function and regulation of this protein in SubtiPathways:
nrgB
MW, pI 12 kDa, 8.825
Gene length, protein length 348 bp, 116 aa
Immediate neighbours nrgA, bcrC
Sequences Protein DNA DNA_with_flanks
Genetic context
NrgB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NrgB expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: P(II) protein family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by ammonium limitation
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References

Reviews

Cheng-Cai Zhang, Cong-Zhao Zhou
ATPase as a switch in P(II) signal transduction.
Proc Natl Acad Sci U S A: 2013, 110(32);12863-4
[PubMed:23847201] [WorldCat.org] [DOI] (I p)

Karl Forchhammer
P(II) signal transducers: novel functional and structural insights.
Trends Microbiol: 2008, 16(2);65-72
[PubMed:18182294] [WorldCat.org] [DOI] (P p)

T Arcondéguy, R Jack, M Merrick
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Microbiol Mol Biol Rev: 2001, 65(1);80-105
[PubMed:11238986] [WorldCat.org] [DOI] (P p)

G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624] [WorldCat.org] [DOI] (P p)


Original publications