Difference between revisions of "Tpi"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 4265 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 4265 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 5061 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 5061 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2920 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1493 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2091 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:''' GP700 (cat), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
 
* '''Mutant:''' GP700 (cat), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
  

Revision as of 14:15, 17 April 2014

  • Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme

Gene name tpi
Synonyms tpiA
Essential no
Product triosephosphate isomerase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: tpi
Interactions involving this protein in SubtInteract: Tpi
Metabolic function and regulation of this protein in SubtiPathways:
tpi
MW, pI 26,9 kDa, 4.79
Gene length, protein length 759 bp, 253 amino acids
Immediate neighbours pgm, pgk
Sequences Protein DNA DNA_with_flanks
Genetic context
Tpi context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tpi expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
  • Protein family: triosephosphate isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylation on Ser-213 PubMed
  • Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed

Database entries

  • Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 4265 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 5061 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2920 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1493 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2091 PubMed

Biological materials

  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References