Difference between revisions of "LysA"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 627 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 627 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5990 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1660 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5360 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A615 ( ''lysA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A615&Search=1A615 BGSC]
 
** 1A615 ( ''lysA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A615&Search=1A615 BGSC]

Revision as of 14:09, 17 April 2014

  • Description: diaminopimelate decarboxylase

Gene name lysA
Synonyms
Essential no
Product diaminopimelate decarboxylase
Function biosynthesis of lysine
Gene expression levels in SubtiExpress: lysA
Metabolic function and regulation of this protein in SubtiPathways:
lysA
MW, pI 48 kDa, 5.007
Gene length, protein length 1317 bp, 439 aa
Immediate neighbours ypuA, spoVAF
Sequences Protein DNA DNA_with_flanks
Genetic context
LysA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LysA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

  • Locus tag: BSU23380

Phenotypes of a mutant

  • auxotrophic for lysine PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Meso-2,6-diaminoheptanedioate = L-lysine + CO2 (according to Swiss-Prot)
  • Protein family: Orn/Lys/Arg decarboxylase class-II family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 627 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5990 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1660 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5360 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References