Difference between revisions of "Ald"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 15145 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 {{PubMed|21395229}}
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** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 15279 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''
 
* '''Mutant:'''
  

Revision as of 14:02, 17 April 2014

  • Description: L-alanine dehydrogenase

Gene name ald
Synonyms spoVN
Essential no
Product L-alanine dehydrogenase
Function alanine utilization
Gene expression levels in SubtiExpress: ald
Metabolic function and regulation of this protein in SubtiPathways:
ald
MW, pI 39 kDa, 5.125
Gene length, protein length 1134 bp, 378 aa
Immediate neighbours adeR, yukJ
Sequences Protein DNA DNA_with_flanks
Genetic context
Ald context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Ald expression.png



















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AdeR regulon

The gene

Basic information

  • Locus tag: BSU31930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH (according to Swiss-Prot)
  • Protein family: AlaDH/PNT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)


  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 15145 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 15279 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed