BkdAA

• Description: 2-oxoisovalerate dehydrogenase (E1 alpha subunit)
  
  

Gene name	bkdAA
Synonyms	bfmBAA, bfmB1a, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E1 alpha subunit)
Function	utilization of branched-chain keto acids
Gene expression levels in SubtiExpress: bkdAA
Metabolic function and regulation of this protein in SubtiPathways: bkdAA
MW, pI	36 kDa, 4.778
Gene length, protein length	990 bp, 330 aa
Immediate neighbours	bkdAB, lpdV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

BkdR regulon, CodY regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU24050

Database entries

• BsubCyc: BSU24050

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: BCKDHA family (according to Swiss-Prot)

• Paralogous protein(s): AcoA, PdhA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• BsubCyc: BSU24050

• Structure: 1UMB (from Thermus thermophilus, 42% identity, 57% similarity) PubMed

• UniProt: P37940

• KEGG entry: [3]

• E.C. number: 1.2.4.4

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Expression browser: bkdAA PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 366 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 932 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 291 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 884 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: