Kbl

• Description: 2-amino-3-ketobutyrate CoA ligase
  
  

Gene name	kbl
Synonyms
Essential	no
Product	2-amino-3-ketobutyrate CoA ligase
Function	threonine utilization
Gene expression levels in SubtiExpress: kbl
Metabolic function and regulation of this protein in SubtiPathways: kbl
MW, pI	43 kDa, 6.25
Gene length, protein length	1176 bp, 392 aa
Immediate neighbours	tdh, ymcB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

The gene

Basic information

• Locus tag: BSU17000

Database entries

• BsubCyc: BSU17000

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate (according to Swiss-Prot)

• Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • pyridoxal-phosphate (according to Swiss-Prot)

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU17000

• Structure: 1FC4 (from Escherichia coli, 40% identity, 60% similarity) PubMed

• UniProt: O31777

• KEGG entry: [2]

• E.C. number: 2.3.1.29

Expression and regulation

• Operon:

• Expression browser: kbl PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1998 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

A Schmidt, J Sivaraman, Y Li, R Larocque, J A Barbosa, C Smith, A Matte, J D Schrag, M Cygler
Three-dimensional structure of 2-amino-3-ketobutyrate CoA ligase from Escherichia coli complexed with a PLP-substrate intermediate: inferred reaction mechanism.
Biochemistry: 2001, 40(17);5151-60
[PubMed:11318637] [WorldCat.org] [DOI] (P p)