Difference between revisions of "DfrA"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 203 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 10:07, 17 April 2014
- Description: dihydrofolate reductase
Gene name | dfrA |
Synonyms | tmp |
Essential | yes PubMed |
Product | dihydrofolate reductase |
Function | biosynthesis of folate |
Gene expression levels in SubtiExpress: dfrA | |
Interactions involving this protein in SubtInteract: DfrA | |
Metabolic function and regulation of this protein in SubtiPathways: dfrA | |
MW, pI | 19 kDa, 5.219 |
Gene length, protein length | 504 bp, 168 aa |
Immediate neighbours | ypkP, thyB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis of cofactors, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU21810
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU21810
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)
- Protein family: spmB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU21810
- Structure: 1ZDR (Geobacillus stearothermophilus)
- UniProt: P11045
- KEGG entry: [2]
- E.C. number: 1.5.1.3
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (complex medium with amino acids, without glucose): 203 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kevin Francis, Vanja Stojkovic, Amnon Kohen
Preservation of protein dynamics in dihydrofolate reductase evolution.
J Biol Chem: 2013, 288(50);35961-8
[PubMed:24158440]
[WorldCat.org]
[DOI]
(I p)
M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350]
[WorldCat.org]
[DOI]
(P p)
T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568]
[WorldCat.org]
[DOI]
(P p)
T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144]
[WorldCat.org]
[DOI]
(P p)