Difference between revisions of "RpoY"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** translation of [[YkzG]] and [[RnjA]] is coupled, and this coupling is required for efficient expression of [[rnjA|RNase J1]] {{PubMed|24187087}}
 
** translation of [[YkzG]] and [[RnjA]] is coupled, and this coupling is required for efficient expression of [[rnjA|RNase J1]] {{PubMed|24187087}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 443 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 10:04, 17 April 2014

Gene name ykzG
Synonyms
Essential no
Product epsilon subunit of RNA polymerase
Function control of RNA polymerase activity
Gene expression levels in SubtiExpress: ykzG
MW, pI 8 kDa, 4.605
Gene length, protein length 207 bp, 69 aa
Immediate neighbours rnjA, ykrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkzG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkzG expression.png















Categories containing this gene/protein

transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein is sometimes annotated as omega1 subunit of RNA polymerase, however, there is no experimental evidence in support of this ("it is unrelated in sequence to ω/Rpb6 from the eubacteria, archaea and eukaryotes, unpublished data suggests it does not bind at the same site as ω/Rpb6 subunits bind their cognate RNAPs, and therefore it is unlikely to function as a true ω subunit") PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0356 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of RNase J1 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 443 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP420 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ailar Jamalli, Agnès Hébert, Léna Zig, Harald Putzer
Control of expression of the RNases J1 and J2 in Bacillus subtilis.
J Bacteriol: 2014, 196(2);318-24
[PubMed:24187087] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)