Difference between revisions of "MsrB"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 309 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 10:00, 17 April 2014
- Description: peptide methionine sulfoxide reductase
Gene name | msrB |
Synonyms | yppQ |
Essential | |
Product | peptide methionine sulfoxide reductase |
Function | regeneration of methionine and restoration of protein function after oxidative damage |
Gene expression levels in SubtiExpress: msrB | |
MW, pI | 16 kDa, 5.842 |
Gene length, protein length | 429 bp, 143 aa |
Immediate neighbours | ypqP, msrA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
resistance against oxidative and electrophile stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU21680
Phenotypes of a mutant
Database entries
- BsubCyc: BSU21680
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU21680
- Structure: 1XM0
- UniProt: P54155
- KEGG entry: [3]
- E.C. number: 1.8.4.12
Additional information
Expression and regulation
- Regulation: induced by oxidative stress (Spx)
- Regulatory mechanism: Spx: oxidized Spx binds RpoA and enhances msrA-msrB operon transcription PubMed
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 309 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
CongHui You, Agnieszka Sekowska, Olivera Francetic, Isabelle Martin-Verstraete, YiPing Wang, Antoine Danchin
Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis.
BMC Microbiol: 2008, 8;128
[PubMed:18662407]
[WorldCat.org]
[DOI]
(I e)