Difference between revisions of "RocD"
Line 125: | Line 125: | ||
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 337 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 113714 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:57, 17 April 2014
- Description: ornithine transaminase
Gene name | rocD |
Synonyms | |
Essential | no |
Product | ornithine transaminase |
Function | arginine, ornithine and citrulline utilization |
Gene expression levels in SubtiExpress: rocD | |
Metabolic function and regulation of this protein in SubtiPathways: rocD | |
MW, pI | 43 kDa, 4.894 |
Gene length, protein length | 1203 bp, 401 aa |
Immediate neighbours | rocE, rocR |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU40340
Phenotypes of a mutant
Database entries
- BsubCyc: BSU40340
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid (according to Swiss-Prot)
- Protein family: OAT subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU40340
- Structure: 3RUY (from B. anthracis)
- UniProt: P38021
- KEGG entry: [3]
- E.C. number: 2.6.1.13
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP656, aphA3, available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Adrienne Zaprasis, Tamara Hoffmann, Guido Wünsche, Lope A Flórez, Jörg Stülke, Erhard Bremer
Mutational activation of the RocR activator and of a cryptic rocDEF promoter bypass loss of the initial steps of proline biosynthesis in Bacillus subtilis.
Environ Microbiol: 2014, 16(3);701-17
[PubMed:23869754]
[WorldCat.org]
[DOI]
(I p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455]
[WorldCat.org]
[DOI]
(P p)
R Gardan, G Rapoport, M Débarbouillé
Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.
J Mol Biol: 1995, 249(5);843-56
[PubMed:7540694]
[WorldCat.org]
[DOI]
(P p)