Difference between revisions of "AsnB"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 289 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 3794 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:56, 17 April 2014

  • Description: asparagine synthase (glutamine-hydrolysing)

Gene name asnB
Synonyms asn
Essential no
Product asparagine synthase (glutamine-hydrolysing))
Function biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnB
Metabolic function and regulation of this protein in SubtiPathways:
asnB
MW, pI 72 kDa, 5.615
Gene length, protein length 1896 bp, 632 aa
Immediate neighbours ytnA, metK
Sequences Protein DNA DNA_with_flanks
Genetic context
AsnB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AsnB expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU30540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
  • Protein family: asparagine synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 289 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3794 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)