Difference between revisions of "AsnB"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 289 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3794 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:56, 17 April 2014
- Description: asparagine synthase (glutamine-hydrolysing)
Gene name | asnB |
Synonyms | asn |
Essential | no |
Product | asparagine synthase (glutamine-hydrolysing)) |
Function | biosynthesis of asparagine |
Gene expression levels in SubtiExpress: asnB | |
Metabolic function and regulation of this protein in SubtiPathways: asnB | |
MW, pI | 72 kDa, 5.615 |
Gene length, protein length | 1896 bp, 632 aa |
Immediate neighbours | ytnA, metK |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30540
Phenotypes of a mutant
Database entries
- BsubCyc: BSU30540
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
- Protein family: asparagine synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU30540
- Structure:
- UniProt: P54420
- KEGG entry: [3]
- E.C. number: 6.3.5.4
Additional information
Expression and regulation
- Sigma factor:
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721]
[WorldCat.org]
[DOI]
(P p)