NagP

• Description: N-acetylglucosamine-specific phosphotransferase system, EIICB of the PTS
  
  

Gene name	nagP
Synonyms	yflF
Essential	no
Product	N-acetylglucosamine-specific PTS, EIICB
Function	N-acetylglucosamine uptake and phosphorylation
Gene expression levels in SubtiExpress: nagP
Metabolic function and regulation of this protein in SubtiPathways: nagP
MW, pI	48 kDa, 7.127
Gene length, protein length	1356 bp, 452 aa
Immediate neighbours	yflG, ltaS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

NagR regulon

The gene

Basic information

• Locus tag: BSU07700

Phenotypes of a mutant

• no growth on N-acetylglucosamine PubMed

Database entries

• BsubCyc: BSU07700

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, glucose permease (Glc) family PubMed

• Paralogous protein(s): GamP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• BsubCyc: BSU07700

• Structure:

• UniProt: O34521

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: nagP PubMed

• Expression browser: nagP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yanfeng Liu, Long Liu, Hyun-dong Shin, Rachel R Chen, Jianghua Li, Guocheng Du, Jian Chen
Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine.
Metab Eng: 2013, 19;107-15
[PubMed:23876412] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Ralph Bertram, Sébastien Rigali, Natalie Wood, Andrzej T Lulko, Oscar P Kuipers, Fritz Titgemeyer
Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.
J Bacteriol: 2011, 193(14);3525-36
[PubMed:21602348] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)