Difference between revisions of "BdhA"

From SubtiWiki
Jump to: navigation, search
Line 38: Line 38:
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[carbon core metabolism]]}},
 
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[phosphoproteins]]}}
+
{{SubtiWiki category|[[phosphoproteins]]}},
 +
[[most abundant proteins]]
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 58: Line 59:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 76: Line 74:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
 
** phosphorylated on Arg-13 {{PubMed|22517742}}
 
** phosphorylated on Arg-13 {{PubMed|22517742}}
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 116: Line 114:
 
* '''Additional information:'''
 
* '''Additional information:'''
 
** The mRNA has a long (268 nt) untranslated leader region {{PubMed|23576037}}
 
** The mRNA has a long (268 nt) untranslated leader region {{PubMed|23576037}}
 +
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
  
 
=Biological materials =
 
=Biological materials =
Line 136: Line 135:
  
 
=References=
 
=References=
<pubmed>18820069 22361854 22517742 22178965 20817675 23576037 </pubmed>
+
<pubmed>18820069 22361854 22517742 22178965 20817675 23576037 15378759</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:16, 5 March 2014

  • Description: acetoine/ butanediol dehydrogenase

Gene name bdhA
Synonyms ydjL
Essential no
Product acetoine/ butanediol dehydrogenase
Function overflow metabolism, fermentation
Gene expression levels in SubtiExpress: bdhA
MW, pI 37 kDa, 4.805
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours iolT, ydjM
Sequences Protein DNA DNA_with_flanks
Genetic context
YdjL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BdhA expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU06240

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: formation of butanediol from acetoin PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-13 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: bdhA (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Rafael R de Oliveira, Wayne L Nicholson
The LysR-type transcriptional regulator (LTTR) AlsR indirectly regulates expression of the Bacillus subtilis bdhA gene encoding 2,3-butanediol dehydrogenase.
Appl Microbiol Biotechnol: 2013, 97(16);7307-16
[PubMed:23576037] [WorldCat.org] [DOI] (I p)

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Ranjita Biswas, Masaru Yamaoka, Hideki Nakayama, Takashi Kondo, Ken-ichi Yoshida, Virendra S Bisaria, Akihiko Kondo
Enhanced production of 2,3-butanediol by engineered Bacillus subtilis.
Appl Microbiol Biotechnol: 2012, 94(3);651-8
[PubMed:22361854] [WorldCat.org] [DOI] (I p)

Claudia Frädrich, Anika March, Kerstin Fiege, Anja Hartmann, Dieter Jahn, Elisabeth Härtig
The transcription factor AlsR binds and regulates the promoter of the alsSD operon responsible for acetoin formation in Bacillus subtilis.
J Bacteriol: 2012, 194(5);1100-12
[PubMed:22178965] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Wayne L Nicholson
The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase.
Appl Environ Microbiol: 2008, 74(22);6832-8
[PubMed:18820069] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)