Difference between revisions of "OpuBC"

From SubtiWiki
Jump to: navigation, search
Line 39: Line 39:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
Line 51: Line 47:
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
+
{{SubtiWiki regulon|[[GbsR regulon]]}},
 +
{{SubtiWiki regulon|[[OpcR regulon]]}}
  
 
=The gene=
 
=The gene=
Line 118: Line 115:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=opuBC_3460503_3461423_-1 opuBC] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=opuBC_3460503_3461423_-1 opuBC] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|10216873}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10216873}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 126: Line 123:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[GbsR]]: transcription repression {{PubMed|22408163}}
 
** [[GbsR]]: transcription repression {{PubMed|22408163}}
 +
** [[OpcR]]: transcription repression {{PubMed|23960087}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 148: Line 146:
  
 
=References=
 
=References=
'''Additional publications:''' {{PubMed|22408163,21366542,21658392}}
+
 
<pubmed>10092453,9925583,10216873, 21296969 </pubmed>
+
<pubmed>22408163,21366542,21658392 10092453,9925583,10216873, 21296969 23960087</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 07:53, 22 August 2013

Gene name opuBC
Synonyms proX
Essential no
Product choline ABC transporter (binding protein)
Function compatible solute transport
Gene expression levels in SubtiExpress: opuBC
Interactions involving this protein in SubtInteract: OpuBC
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 34 kDa, 6.618
Gene length, protein length 918 bp, 306 aa
Immediate neighbours opuBD, opuBB
Sequences Protein DNA DNA_with_flanks
Genetic context
OpuBC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OpuBC expression.png















Categories containing this gene/protein

ABC transporters, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

GbsR regulon, OpcR regulon

The gene

Basic information

  • Locus tag: BSU33710

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): OpuCC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Chun-Hao Lee, Tien-Yu Wu, Gwo-Chyuan Shaw
Involvement of OpcR, a GbsR-type transcriptional regulator, in negative regulation of two evolutionarily closely related choline uptake genes in Bacillus subtilis.
Microbiology (Reading): 2013, 159(Pt 10);2087-2096
[PubMed:23960087] [WorldCat.org] [DOI] (I p)

Gabriele Nau-Wagner, Daniela Opper, Anne Rolbetzki, Jens Boch, Bettina Kempf, Tamara Hoffmann, Erhard Bremer
Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
J Bacteriol: 2012, 194(10);2703-14
[PubMed:22408163] [WorldCat.org] [DOI] (I p)

Marco Pittelkow, Britta Tschapek, Sander H J Smits, Lutz Schmitt, Erhard Bremer
The crystal structure of the substrate-binding protein OpuBC from Bacillus subtilis in complex with choline.
J Mol Biol: 2011, 411(1);53-67
[PubMed:21658392] [WorldCat.org] [DOI] (I p)

Yang Du, Wei-Wei Shi, Yong-Xing He, Yi-Hu Yang, Cong-Zhao Zhou, Yuxing Chen
Structures of the substrate-binding protein provide insights into the multiple compatible solute binding specificities of the Bacillus subtilis ABC transporter OpuC.
Biochem J: 2011, 436(2);283-9
[PubMed:21366542] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

R M Kappes, B Kempf, S Kneip, J Boch, J Gade, J Meier-Wagner, E Bremer
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Mol Microbiol: 1999, 32(1);203-16
[PubMed:10216873] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

G Nau-Wagner, J Boch, Le Good JA, E Bremer
High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis.
Appl Environ Microbiol: 1999, 65(2);560-8
[PubMed:9925583] [WorldCat.org] [DOI] (P p)