Difference between revisions of "PGP1870"

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* ''E.coli'': multicopy plasmid, select for ampicillin
 
* ''E.coli'': multicopy plasmid, select for ampicillin
 
* ''B. subtilis'': no ori, select for spectinomycin
 
* ''B. subtilis'': no ori, select for spectinomycin
== ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!! Use [[pBP43]] instead! ==
 
  
 
'''Hints for the usage'''
 
'''Hints for the usage'''

Revision as of 16:57, 3 June 2013

pGP1870: click to enlarge

General comments

Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

  • E.coli: multicopy plasmid, select for ampicillin
  • B. subtilis: no ori, select for spectinomycin

Hints for the usage

  • amplify the last 600bp of your gene of interest,
  • skip the Stopp-Codon of the gene in the reverse primer
  • take care that the integration is in-frame
  • do not use the HindIII site for cloning, as GFP is inserted via this restriction site

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