Difference between revisions of "TetB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to tetracyclin
 
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to tetracyclin
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU40770 tetB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40770 tetB]
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.537   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.537   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaP]]'', ''[[tetL]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaP]]'', ''[[tetL]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16114&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40770 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:tetB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:tetB_context.gif]]

Revision as of 14:20, 13 May 2013

  • Description: multifunctional tetracycline/Na+ resistance protein

Gene name tetB
Synonyms tetA, tetA(L)
Essential no
Product multifunctional tetracycline/Na+ resistance protein
Function resistance to tetracyclin
Gene expression levels in SubtiExpress: tetB
MW, pI 49 kDa, 9.537
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours yyaP, tetL
Sequences Protein DNA Advanced_DNA
Genetic context
TetB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TetB expression.png


























Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: TCR/tet family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Your additional remarks

References

W Wang, A A Guffanti, Y Wei, M Ito, T A Krulwich
Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K(+) acquisition as well as in Na(+), alkali, and tetracycline resistance.
J Bacteriol: 2000, 182(8);2088-95
[PubMed:10735849] [WorldCat.org] [DOI] (P p)

S J Stasinopoulos, G A Farr, D H Bechhofer
Bacillus subtilis tetA(L) gene expression: evidence for regulation by translational reinitiation.
Mol Microbiol: 1998, 30(5);923-32
[PubMed:9988470] [WorldCat.org] [DOI] (P p)

J Cheng, D B Hicks, T A Krulwich
The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange.
Proc Natl Acad Sci U S A: 1996, 93(25);14446-51
[PubMed:8962071] [WorldCat.org] [DOI] (P p)

J Cheng, A A Guffanti, W Wang, T A Krulwich, D H Bechhofer
Chromosomal tetA(L) gene of Bacillus subtilis: regulation of expression and physiology of a tetA(L) deletion strain.
J Bacteriol: 1996, 178(10);2853-60
[PubMed:8631673] [WorldCat.org] [DOI] (P p)

J Cheng, K Baldwin, A A Guffanti, T A Krulwich
Na+/H+ antiport activity conferred by Bacillus subtilis tetA(L), a 5' truncation product of tetA(L), and related plasmid genes upon Escherichia coli.
Antimicrob Agents Chemother: 1996, 40(4);852-7
[PubMed:8849239] [WorldCat.org] [DOI] (P p)

R Sakaguchi, H Amano, K Shishido
Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.
Biochim Biophys Acta: 1988, 950(3);441-4
[PubMed:2844262] [WorldCat.org] [DOI] (P p)