Difference between revisions of "NagP"

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* '''Description:''' N-acetylglucosamine-specific phosphotransferase system, EIICB of the [[PTS]]<br/><br/>
 
 
 
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Revision as of 11:04, 13 August 2012

  • Description: N-acetylglucosamine-specific phosphotransferase system, EIICB of the PTS

Gene name nagP
Synonyms yflF
Essential no
Product N-acetylglucosamine-specific PTS, EIICB
Function N-acetylglucosamine uptake and phosphorylation
Gene expression levels in SubtiExpress: nagP
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 48 kDa, 7.127
Gene length, protein length 1356 bp, 452 aa
Immediate neighbours yflG, ltaS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NagP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NagP expression.png




























Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

NagR regulon

The gene

Basic information

  • Locus tag: BSU07700

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
  • Protein family: PTS permease, glucose permease (Glc) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • induced in the presence of N-acetylglucosamine (NagR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)