Difference between revisions of "SigM"

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* '''Description:''' [[RNA polymerase]] ECF-type [[sigma factor]] [[SigM]] <br/><br/>
+
* '''Description:''' [[RNA polymerase]] ECF-type [[sigma factor]] [[SigM]], responsible for intrinsic resistance against beta-lactam antibiotics <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || [[RNA polymerase]] ECF-type [[sigma factor]] [[SigM]]
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[RNA polymerase]] ECF-type [[sigma factor]] [[SigM]]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance against cell envelope stress, <br/>oxidative stress and salt stress
+
|style="background:#ABCDEF;" align="center"|'''Function''' || responsible for intrinsic resistance against beta-lactam antibiotics
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigM SigM]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigM SigM]
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<br/><br/><br/><br/>
 
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{{SubtiWiki category|[[transcription]]}},
 
{{SubtiWiki category|[[transcription]]}},
 
{{SubtiWiki category|[[sigma factors and their control]]}},
 
{{SubtiWiki category|[[sigma factors and their control]]}},
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}
+
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
 +
{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl {{PubMed|10216858}}
 
* SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl {{PubMed|10216858}}
 
* ''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts {{PubMed|10216858}}
 
* ''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts {{PubMed|10216858}}
 +
* increased sensitivity towards beta-lactam antibiotics {{PubMed|22211522}}
  
 
=== Database entries ===
 
=== Database entries ===
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<pubmed>18179421,17675383,17434969,</pubmed>
 
<pubmed>18179421,17675383,17434969,</pubmed>
 
==Other original publications==
 
==Other original publications==
<pubmed>22362028 18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214 ,19465659  19745567 10216858 20057163 </pubmed>
+
<pubmed>22362028 18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214 ,19465659  19745567 10216858 20057163 22211522 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:44, 21 July 2012

Gene name sigM
Synonyms yhdM
Essential no
Product RNA polymerase ECF-type sigma factor SigM
Function responsible for intrinsic resistance against beta-lactam antibiotics
Interactions involving this protein in SubtInteract: SigM
MW, pI 19 kDa, 6.77
Gene length, protein length 489 bp, 163 aa
Immediate neighbours yhdL, yhdN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigM expression.png
















Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigM regulon

The SigM regulon

The gene

Basic information

  • Locus tag: BSU09520

Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
  • sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
  • increased sensitivity towards beta-lactam antibiotics PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family, ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Additional publications: PubMed

The SigM regulon

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Thorsten Mascher, Anna-Barbara Hachmann, John D Helmann
Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors.
J Bacteriol: 2007, 189(19);6919-27
[PubMed:17675383] [WorldCat.org] [DOI] (P p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Other original publications