Difference between revisions of "OpuE"

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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:'''
+
* '''Protein family:''' solute symporter family
  
 
* '''Paralogous protein(s):''' [[PutP]]
 
* '''Paralogous protein(s):''' [[PutP]]

Revision as of 08:29, 14 June 2012

  • Description: proline transporter

Gene name opuE
Synonyms yerK
Essential no
Product proline transporter
Function compatible solute transport
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 53 kDa, 9.815
Gene length, protein length 1476 bp, 492 aa
Immediate neighbours sapB, gatC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
OpuE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OpuE expression.png




























Categories containing this gene/protein

general stress proteins (controlled by SigB), transporters/ other, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU06660

Phenotypes of a mutant

  • strong growth disadvantage in high-salinity minimal media lacking proline PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: solute symporter family
  • Paralogous protein(s): PutP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Tamara Hoffmann, Carsten von Blohn, Agnieszka Stanek, Susanne Moses, Helena Barzantny, Erhard Bremer
Synthesis, release, and recapture of compatible solute proline by osmotically stressed Bacillus subtilis cells.
Appl Environ Microbiol: 2012, 78(16);5753-62
[PubMed:22685134] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

F Spiegelhalter, E Bremer
Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A- and sigma B-dependent stress-responsive promoters.
Mol Microbiol: 1998, 29(1);285-96
[PubMed:9701821] [WorldCat.org] [DOI] (P p)

C von Blohn, B Kempf, R M Kappes, E Bremer
Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.
Mol Microbiol: 1997, 25(1);175-87
[PubMed:11902719] [WorldCat.org] [DOI] (P p)