Difference between revisions of "TrpS"

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|colspan="2" | '''Genetic context''' <br/> [[Image:trpS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:trpS_context.gif]]
 
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpS_1218113_1219105_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:trpS_expression.png|500px]]
 
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Revision as of 17:29, 18 April 2012

  • Description: tryptophanyl-tRNA synthetase

Gene name trpS
Synonyms
Essential yes PubMed
Product tryptophanyl-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 37 kDa, 6.542
Gene length, protein length 990 bp, 330 aa
Immediate neighbours yjbA, oppA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TrpS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrpS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU11420

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3PRH (Val144Pro mutant) PubMed
    • 1BM2 (complex with tryptophan, Geobacillus stearothermophilus)
    • 1MAW (complex with ATP, Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
    • T-box: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alicja K Antonczak, Zuzana Simova, Isaac T Yonemoto, Matthias Bochtler, Anna Piasecka, Honorata Czapinska, Andrea Brancale, Eric M Tippmann
Importance of single molecular determinants in the fidelity of expanded genetic codes.
Proc Natl Acad Sci U S A: 2011, 108(4);1320-5
[PubMed:21224416] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A I Lee, J P Sarsero, C Yanofsky
A temperature-sensitive trpS mutation interferes with trp RNA-binding attenuation protein (TRAP) regulation of trp gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(22);6518-24
[PubMed:8932308] [WorldCat.org] [DOI] (P p)

C Condon, M Grunberg-Manago, H Putzer
Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis.
Biochimie: 1996, 78(6);381-9
[PubMed:8915527] [WorldCat.org] [DOI] (P p)