Difference between revisions of "DacB"

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(Extended information on the protein)
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=== Additional information===
 
=== Additional information===
 
 
  
  
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:''' modifies degree of cross-linking of glycan strands in peptidoglycan
  
 
* '''Protein family:''' peptidase S11 family (according to Swiss-Prot)
 
* '''Protein family:''' peptidase S11 family (according to Swiss-Prot)
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=References=
 
=References=
<pubmed>10498740,9864321,1548223,7642500,14731276,19542328 ,7528199 15699190 </pubmed>
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<pubmed>10498740,9864321,1548223,7642500,14731276,19542328 ,7528199 15699190 22123250 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:04, 2 December 2011

Gene name dacB
Synonyms
Essential no
Product penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function carboxypeptidase
MW, pI 42 kDa, 9.725
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours spmA, ypuI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DacB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU23190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan
  • Protein family: peptidase S11 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3MFD (beta-lactamase superfamily domain)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pengfei Zhang, Stacy Thomas, Yong-qing Li, Peter Setlow
Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination.
J Bacteriol: 2012, 194(3);646-52
[PubMed:22123250] [WorldCat.org] [DOI] (I p)

Ralf Moeller, Peter Setlow, Günther Reitz, Wayne L Nicholson
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation.
Appl Environ Microbiol: 2009, 75(16);5202-8
[PubMed:19542328] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

D L Popham, B Illades-Aguiar, P Setlow
The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration.
J Bacteriol: 1995, 177(16);4721-9
[PubMed:7642500] [WorldCat.org] [DOI] (P p)

E B Simpson, T W Hancock, C E Buchanan
Transcriptional control of dacB, which encodes a major sporulation-specific penicillin-binding protein.
J Bacteriol: 1994, 176(24);7767-9
[PubMed:7528199] [WorldCat.org] [DOI] (P p)

C E Buchanan, M L Ling
Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis.
J Bacteriol: 1992, 174(6);1717-25
[PubMed:1548223] [WorldCat.org] [DOI] (P p)