Difference between revisions of "SubtInteract"

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(Suspected hub proteins potentially involved in a large number of interactions)
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=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
 
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
  
==Suspected hub proteins potentially involved in a large number of interactions==
+
==Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)==
 +
* [[FruA]], [SwrC], [[XhlA]], [[YhaP]], [[YhgE]], [[YkcC]], [[YqfF]], [[CsbC]], [[CssS]], [[FliZ]], [[MreD]], [[PpsC]], [[RacA]], [[Smc]], [[YclI]], [[YdeL]], [[YdgH]], [[YdbI]], [[YesS]], [[YkoT]], [[YopZ]], [[YqbD]], [[YtdP]], [[YueB]], [[YwqJ]], [[YyxA]]
 
<pubmed> 21630458</pubmed>
 
<pubmed> 21630458</pubmed>
  
 
==Important publications==
 
==Important publications==
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>

Revision as of 19:49, 16 June 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

DNA replication: the replisome

transcription: RNA polymerase

translation: the ribosome

synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln

RNA processing and degradation: the RNA degradosome

general stress response: the stressosome

cell division: the divisome

DNA uptake: the pseudopilus PubMed

metabolism: the metabolons of glycolysis and the TCA cycle PubMed

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)


Important publications

Agnès Vendeville, Damien Larivière, Eric Fourmentin
An inventory of the bacterial macromolecular components and their spatial organization.
FEMS Microbiol Rev: 2011, 35(2);395-414
[PubMed:20969605] [WorldCat.org] [DOI] (I p)

Mike P Williamson, Michael J Sutcliffe
Protein-protein interactions.
Biochem Soc Trans: 2010, 38(4);875-8
[PubMed:20658969] [WorldCat.org] [DOI] (I p)

Patrick Amar, Guillaume Legent, Michel Thellier, Camille Ripoll, Gilles Bernot, Thomas Nystrom, Milton H Saier, Vic Norris
A stochastic automaton shows how enzyme assemblies may contribute to metabolic efficiency.
BMC Syst Biol: 2008, 2;27
[PubMed:18366733] [WorldCat.org] [DOI] (I e)

Adam Brymora, Valentina A Valova, Phillip J Robinson
Protein-protein interactions identified by pull-down experiments and mass spectrometry.
Curr Protoc Cell Biol: 2004, Chapter 17;Unit 17.5
[PubMed:18228443] [WorldCat.org] [DOI] (I p)

Hongtao Guan, Endre Kiss-Toth
Advanced technologies for studies on protein interactomes.
Adv Biochem Eng Biotechnol: 2008, 110;1-24
[PubMed:18219467] [WorldCat.org] [DOI] (I p)

Vincent Collura, Guillaume Boissy
From protein-protein complexes to interactomics.
Subcell Biochem: 2007, 43;135-83
[PubMed:17953394] [WorldCat.org] [DOI] (P p)

Philippe Noirot, Marie-Françoise Noirot-Gros
Protein interaction networks in bacteria.
Curr Opin Microbiol: 2004, 7(5);505-12
[PubMed:15451506] [WorldCat.org] [DOI] (P p)

Barry Causier
Studying the interactome with the yeast two-hybrid system and mass spectrometry.
Mass Spectrom Rev: 2004, 23(5);350-67
[PubMed:15264234] [WorldCat.org] [DOI] (P p)

Andrzej Dziembowski, Bertrand Séraphin
Recent developments in the analysis of protein complexes.
FEBS Lett: 2004, 556(1-3);1-6
[PubMed:14706816] [WorldCat.org] [DOI] (P p)

Alfonso Valencia, Florencio Pazos
Computational methods for the prediction of protein interactions.
Curr Opin Struct Biol: 2002, 12(3);368-73
[PubMed:12127457] [WorldCat.org] [DOI] (P p)

Peter Uetz
Two-hybrid arrays.
Curr Opin Chem Biol: 2002, 6(1);57-62
[PubMed:11827824] [WorldCat.org] [DOI] (P p)

P Legrain, J Wojcik, J M Gauthier
Protein--protein interaction maps: a lead towards cellular functions.
Trends Genet: 2001, 17(6);346-52
[PubMed:11377797] [WorldCat.org] [DOI] (P p)

C L Tucker, J F Gera, P Uetz
Towards an understanding of complex protein networks.
Trends Cell Biol: 2001, 11(3);102-6
[PubMed:11306254] [WorldCat.org] [DOI] (P p)

P Uetz, R E Hughes
Systematic and large-scale two-hybrid screens.
Curr Opin Microbiol: 2000, 3(3);303-8
[PubMed:10851163] [WorldCat.org] [DOI] (P p)

Z Chen, M Han
Building a protein interaction map: research in the post-genome era.
Bioessays: 2000, 22(6);503-6
[PubMed:10842303] [WorldCat.org] [DOI] (P p)

P A Srere
Macromolecular interactions: tracing the roots.
Trends Biochem Sci: 2000, 25(3);150-3
[PubMed:10694888] [WorldCat.org] [DOI] (P p)

P A Srere, J Ovadi
Enzyme-enzyme interactions and their metabolic role.
FEBS Lett: 1990, 268(2);360-4
[PubMed:2200717] [WorldCat.org] [DOI] (P p)

P A Srere
Complexes of sequential metabolic enzymes.
Annu Rev Biochem: 1987, 56;89-124
[PubMed:2441660] [WorldCat.org] [DOI] (P p)