Difference between revisions of "BirA"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
+
=The [[BirA regulon]]:=
 +
* ''[[bioW]]-[[bioA]]-[[bioF]]-[[bioD]]-[[bioB]]-[[bioI]]-[[ytbQ]]''
  
 
=The gene=
 
=The gene=

Revision as of 17:45, 11 December 2010

  • Description: trigger enzyme: transcriptional repressor of the biotin operon / biotin-protein ligase

Gene name birA
Synonyms
Essential yes PubMed
Product trigger enzyme: transcriptional repressor/ biotin-protein ligase
Function regulation of biotin synthesis, addition of biotin to proteins
MW, pI 36 kDa, 6.769
Gene length, protein length 975 bp, 325 aa
Immediate neighbours panB, cca
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BirA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biosynthesis of lipids, biosynthesis of cofactors, transcription factors and their control, trigger enzyme, essential genes

This gene is a member of the following regulons

The BirA regulon:

The gene

Basic information

  • Locus tag: BSU22440

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • transcriptional repressor of the bioW-bioA-bioF-bioD-bioB-bioI-ytbQ operon PubMed and
    • ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] (according to Swiss-Prot)
  • Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Operon and expression

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)