Difference between revisions of "HolB"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[DNA replication]]}},
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{{SubtiWiki category|[[essential genes]]}}
 
=The protein=
 
=The protein=
  

Revision as of 17:19, 30 November 2010

  • Description: DNA polymerase III (delta subunit), part of the replisome

Gene name holB
Synonyms yaaS
Essential yes PubMed
Product DNA polymerase III (delta subunit)
Function replication
MW, pI 37 kDa, 6.334
Gene length, protein length 987 bp, 329 aa
Immediate neighbours yaaR, yaaT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HolB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00310

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

Categories containing this gene/protein

DNA replication, essential genes

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (homogeneous) PubMed

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)